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The open reading frame of GLR1 and TSA1 gene was amplified by PCR from yeast genomic DNA and cloned into the p416 vector with TEF promoter and Cterminal triple hemagglutinin (HA) tag

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Unlike iron - which can induce non-specific oxidative damage through Fenton reactions - or zinc, which contributes to structural and catalytic stability without participating in redox cycling, copper supports tightly regulated electron transfer and dynamic, chaperone-mediated signaling processes via proteins such as Atox1

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